Journal
JOURNAL OF BIOTECHNOLOGY
Volume 148, Issue 4, Pages 204-207Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2010.06.008
Keywords
Thermophilic enzyme; Artificial bio-synthetic pathway; 2-Deoxyribose 5-phosphate; 2-Deoxyriboaldolase
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Six thermophilic enzymes from Thermus thermophilus were used to construct an 'artificial bio-synthetic pathway' for the production of 2-deoxyribose 5-phosphate from fructose. By a simple operation using six recombinant Escherichia coli strains producing the thermophilic enzymes, respectively, fructose was converted to 2-deoxyribose 5-phosphate with a molar yield of 55%. (C) 2010 Elsevier B.V. All rights reserved.
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