Journal
JOURNAL OF BIOTECHNOLOGY
Volume 145, Issue 3, Pages 240-243Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2009.11.022
Keywords
Henry reaction; Nitroalkanes; Aldehyde; Hydrolase; beta-Nitroalkanols
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Nitroalkanes underwent fast additions to a variety of structurally diverse alclehydes under the catalysis of D-aminoacylase in DMSO. The influences of reaction conditions including solvents, temperature, enzyme concentration and molar ratio of substrates were systematically investigated. Seventeen products were obtained in short time with moderate to high yields. It is the first report on hydrolase-catalyzed fast Henry reaction in organic solvent. (C) 2009 Elsevier B.V. All rights reserved.
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