Journal
JOURNAL OF BIOTECHNOLOGY
Volume 145, Issue 1, Pages 17-22Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2009.10.004
Keywords
Suspension arrays; DNA hybridisation; DNA quantification; Microbeads; Flow cytometry; Immobilised oligonucleotides
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Funding
- NBCF through the National Collaborative Breast Cancer Research Grant Program
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A novel format of a multiplex microbead assay that is capable of accurately and reproducibly measuring small changes in 10(8) copies of a nucleic acid is reported herein. The dynamic range of the assay was 32-fold greater than conventional multiplex microbead assays. The assay was modeled mathematically which enabled the accuracy of DNA quantification to be formally analysed using a logical theoretical framework. The assay was also evaluated on RNA extracts taken from stimulated macrophages, to demonstrate that changes in gene expression can be accurately monitored in a biological system. We believe the reproducibility and accuracy of the assay presented herein will be useful for high-throughput, multiplex sensitive detection of nucleic acids over a large dynamic range in a research or diagnostic setting. Crown Copyright (c) 2009 Published by Elsevier B.V. All rights reserved.
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