Journal
JOURNAL OF BIOTECHNOLOGY
Volume 143, Issue 4, Pages 284-287Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2009.08.009
Keywords
Xylose utilization; Clostridium acetobutylicum; Overexpressing transaldolase; Solvent production
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Funding
- National Basic Research Program of China [973: 2007CB707803]
- National High-tech Research and Development Program of China [863: 2006AA02Z237, 863: 2007AA05Z407]
- Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-YW-G-007]
- Planned Scientific Program of Science and Technology Commission of Shanghai Municipality [08dz1207100]
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Clostridium acetobutylicum ATCC 824 was metabolically engineered for improved xylose utilization. The gene talA, which encodes transaldolase from Escherichia coli K-12, was cloned and overexpressed in C. acetobutylicum ATCC 824. Compared with C. acetobutylicum ATCC 824 (824-WT), the transformant bearing the E. coli talA gene (824-TAL) showed improved ability on xylose utilization and solvents production using xylose as the sole carbon source. During the fermentation of xylose and glucose mixtures with three xylose/glucose ratios (approximately 1:2, 1:1 and 2:1), the rate of xylose consumption and final solvents titers of 824-TAL were all higher than those of 824-WT, despite glucose repression on xylose uptake still existing. These results suggest that the insufficiency of transaldolase in the pentose phosphate pathway (PPP) of C. acetobutylicum is one of the bottlenecks for xylose metabolism and therefore, overexpressing the gene encoding transaldolase is able to improve xylose utilization and solvent production. (C) 2009 Elsevier B.V. All rights reserved.
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