Journal
JOURNAL OF BIOTECHNOLOGY
Volume 142, Issue 2, Pages 179-184Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2009.04.005
Keywords
Horseradish peroxidase (HRP); NanoCeO(2); Biosensor; Hydrogen peroxide
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Funding
- CSIR
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Nanostructured cerium oxide (NanoCeO(2)) film deposited onto indium-tin-oxide (ITO) glass substrate by solution casting has been used for immobilization of horseradish peroxidase (HRP) via physiosorption technique. X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-vis and electrochemical techniques have been utilized for characterization of NanoCeO(2)/ITO electrode and HRP/NanoCeO(2)/ITO bioelectrode. The HRP/NanoCeO(2)/ITO electrode exhibits value of the apparent Michaelis-Menten constant (K-m) as 2.21 mu M, linear regression coefficient as 0.998 and linearity for hydrogen peroxide as 1.0-170 mu M obtained using electrochemical response measurements. Besides this, HRP/NanoCeO(2)/ITO bioelectrode can be used about 20 times and is stable for 5 weeks at 4 degrees C. The results of photo-response studies carried out on HRP/NanoCeO(2)/ITO bioelectrode indicate reasonable agreement with those obtained using amperometric technique. (C) 2009 Elsevier B.V. All rights reserved.
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