Journal
JOURNAL OF BIOTECHNOLOGY
Volume 141, Issue 3-4, Pages 109-117Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2009.03.014
Keywords
Cerevisiae; Organelle; CalMorph; Morphology; Image processing
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Funding
- Ministry of Education, Science, Sports, and Culture of Japan
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To quantitatively and multidimensionally assess the morphology of subcellular organelles and protein complexes in budding yeast cells, we applied our recently developed image-processing program, CalMorph. In this study, mitochondria, vacuole, endoplasmic reticulum, Golgi body, endosome, spindle pole body, and septin morphology were evaluated. In addition to the originally developed 501 parameters for cell wall Morphology, nuclear DNA, and actin, we proposed an additional 610 parameters for the molphology of subcellular components, resulting in a total of 1111 quantitative parameters to evaluate the morphology of budding yeast. This approach enables one to conduct more detailed phenotypic Studies, which is advantageous in yeast functional genomics. (C) 2009 Elsevier B.V. All rights reserved.
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