Journal
JOURNAL OF BIOTECHNOLOGY
Volume 133, Issue 3, Pages 343-350Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2007.10.012
Keywords
lipase; mathematical model; specificity constant; triacylglycerol; diacylglycerol; monoacylglycerol; sequential reactions
Categories
Ask authors/readers for more resources
We propose a model for the sequential hydrolysis of ester bonds of triacylglycerols by lipases and use it as the basis for an analytical method for determining the relative specificity of the lipase for the various substrates with which it can react, when the substrates occur simultaneously in a single reaction system. We then apply the method to our own data and literature data involving the hydrolysis of triacylglycerols by lipases. Our model is able to fit well to most of the reaction profiles, enabling the estimation of relative specificities. We discuss the limitations and potential applications of our method. (C) 2007 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available