Journal
JOURNAL OF BIOTECHNOLOGY
Volume 136, Issue 1-2, Pages 38-43Publisher
ELSEVIER
DOI: 10.1016/j.jbiotec.2008.04.013
Keywords
whole-genome shotgun; sanger sequencing; sequencing by synthesis; genome assembly; heterozygosity; polymorphism
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Funding
- Province of Trento
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A new approach to sequencing and assembling a highly heterozygous genome, that of grape, species Vitis vinifera cv Pinot Noir, is described. The combining of genome shotgun of paired reads produced by Sanger sequencing and sequencing by synthesis of unpaired reads was shown to be an efficient procedure for decoding a complex genome. About 2 million SNPs and more than a million heterozygous gaps have been identified in the 500Mb genome of grape. More than 91% of the sequence assembled into 58,611 contigs is now anchored to the 19 linkage groups Of V. vinifera. (C) 2008 Elsevier B.V. All rights reserved.
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