Journal
NEURAL REGENERATION RESEARCH
Volume 10, Issue 6, Pages 951-957Publisher
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/1673-5374.158360
Keywords
nerve regeneration; spinal cord injury; rapamycin; Wnt/beta-catenin signaling pathway; apoptosis; caspase-3; brain-derived neurotrophic factor; neuroprotection; loss of neurons; NSFC grants; neural regeneration
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Funding
- National Natural Science Foundation of China [81171799, 81471854]
- China Postdoctoral Science Foundation [2013T60948]
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The Wnt/beta-catenin signaling pathway plays a crucial role in neural development, axonal guidance, neuropathic pain remission and neuronal survival. In this study, we initially examined the effect of rapamycin on the Wnt/beta-catenin signaling pathway after spinal cord injury, by intraperitoneally injecting spinal cord injured rats with rapamycin over 2 days. Western blot analysis and immunofluorescence staining were used to detect the expression levels of beta-catenin protein, caspase-3 protein and brain-derived neurotrophic factor protein, components of the Wnt/beta-catenin signaling pathway. Rapamycin increased the levels of beta-catenin and brain-derived neurotrophic factor in the injured spinal cord, improved the pathological morphology at the injury site, reduced the loss of motor neurons, and promoted motor functional recovery in rats after spinal cord injury. Our experimental findings suggest that the neuroprotective effect of rapamycin intervention is mediated through activation of the Wnt/beta-catenin signaling pathway after spinal cord injury.
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