Journal
JOURNAL OF BIOSCIENCES
Volume 39, Issue 3, Pages 401-413Publisher
INDIAN ACAD SCIENCES
DOI: 10.1007/s12038-014-9432-1
Keywords
Cerebellar Purkinje cells; spermatogenesis; SPOP; miR-145; TD/POZ protein family; testicular seminiferous tubule
Categories
Funding
- National Science Council (Taiwan) [NSC-101-2313-B-034-003]
- University Tunku Abdul Rahman intramural grant [6200/C39]
Ask authors/readers for more resources
The speckle POZ protein, SPOP, is an adaptor of the Cu13-based ubiquitination process, and has been implicated in the carcinogenesis process. Despite recent elucidation of biological functions, regulation of SPOP gene expression has not been reported. In this study, the mRNA levels of the mouse SPOP (mSPOP) gene were first shown to vary noticeably in different tissues. However, the SPOP protein was detected in high abundance only in Purkinje cells of the cerebellum and seminiferous tubule of the testis, echoing previous reports of involvement of ubiquitination in neuron cells and in spermatogenesis. In other mouse tissues and human cancer cell lines analysed, only low SPOP protein levels were detected. The 3'-untranslated regions of both the mSPOP and human SPOP transcripts harbor a conserved putative miR-145 binding site (BS). In some tissues and cell lines, miR-145 and SPOP protein levels were in an inverse relationship suggesting miR-145 regulation. Luciferase assays of deletion and point mutation constructs of the miR-145 BS, and miR-145 induction by serum starvation that resulted in reduced endogenous SPOP levels provided further evidence that miR-145 is likely involved in post-transcriptional regulation of SPOP expression in selected tissues, and possibly with the participation of other miRNA species.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available