Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 117, Issue 3, Pages 379-382Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2013.08.020
Keywords
Gas chromatography; Mass spectrometry; Polyhydroxyalkanoate; 3-Hydroxyalkanoic acid; Homologous
Funding
- National Environment Agency (NEA), Singapore [ETRP 0901 161]
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A gas chromatography-mass spectrometry method for quantification of polyhydroxyalkanoates (PHAs), containing 4-carbon to 16-carbon monomers, even in the absence of standards, was developed. Strong linear correlations existed between PHA carbon number and retention time/response factor (R-2 >= 0.987). Based on the correlations, high recovery values, between 100.5% and 114.3%, were obtained for PHA polymers. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.
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