Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 117, Issue 4, Pages 504-511Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2013.10.001
Keywords
Tip growing cell; Microfluidic; Hydrodynamic trapping; Plant cell; Pollen tube
Funding
- Fonds de recherche du Quebec - Nature et Technologies (FQRNT)
- Concordia Research Chair
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A lab-on-a-chip device with a knot shaped microfluidic network is presented to enable trapping of single pollen grains at the entrances of a series of microchannels. This set-up serves to create identical growth conditions for serially arranged tip growing plant cells such as pollen tubes. The design consists of an inlet to introduce the pollen suspension into the chip, three outlets to evacuate excess medium or cells, a distribution chamber to guide the pollen grains toward the growth microchannels and a serial arrangement of microchannels with different geometries connected to the distribution chamber. These microchannels are to harbor the individual pollen tubes. Two different criteria were established to assess the efficiency and optimize the device: trapping probability and uniformity of fluid flow conditions within the microchannels. The performance of different geometries of the microfluidic network was numerically analyzed and experimentally tested. (c) 2013, The Society for Biotechnology, Japan. All rights reserved.
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