Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 115, Issue 6, Pages 691-694Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2012.12.020
Keywords
Algae; Chlamydomonas reinhardtii; Electroporation; Square electric pulse; Transformation
Funding
- JST
- ALCA
- KAKENHI [23120514, 22380059, 23770172]
- Grants-in-Aid for Scientific Research [23770172, 25840109, 22380059] Funding Source: KAKEN
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Chlamydomonas reinhardtii is widely used to study many biological processes including biofuel production. Here, we present a rapid transformation technique for cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to transformation of other industrially useful algae by optimizing the electric conditions. (C) 2013, The Society for Biotechnology, japan. All rights reserved.
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