Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 114, Issue 5, Pages 546-551Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2012.05.022
Keywords
Liver tissue engineering; Decellularized liver; Vascular tree network; Endothelialized blood vessel; Hepatocyte/spheroid
Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [22360348]
- Grants-in-Aid for Scientific Research [22360348] Funding Source: KAKEN
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The construction of a functional liver-tissue equivalent using tissue engineering is a very important goal because the liver is a central organ in the body. However, the construction of functional organ-scale liver tissue is impossible because it requires a high-density blood vessel network. In this study, we focused on decellularization technology to solve this problem. Decellularized liver tissue with a fine vascular tree network template was obtained using Triton X-100. The distance between each vascular structure was less than 1 mm. Endothelialization of the blood vessel network with human umbilical vein endothelial cells (HUVECs) was successfully performed without any leakage of HUVECs to the outside of the vessel structure. Furthermore, hepatocytes/spheroids could be located around the blood vessel structure. This study indicates that decellularized liver tissue is a potential scaffold for creating a practical liver tissue using tissue engineering technology. (C) 2012. The Society for Biotechnology, Japan. All rights reserved.
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