4.4 Article

Visualizing green oil in live algal cells

Journal

JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 109, Issue 2, Pages 198-201

Publisher

SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2009.08.004

Keywords

BODIPY 505/515; Vital stain; Lipid body; Oleaginous; FACS; Algae

Funding

  1. University of Washington
  2. Allied Minds

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We report here that BODIPY 505/515, a green lipophilic fluorescent dye, serves as an excellent vital stain for the oil-containing lipid bodies of live algal cells. BODIPY 505/515 vital staining can be used in combination with fluorescent activated cell sorting to detect and isolate algal cells possessing high lipid content. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

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