Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 110, Issue 2, Pages 169-175Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2010.01.018
Keywords
Extremobiosphere; Biosurfactant; Mannosylerythritol lipid; Vegetable oil; Pseudozyma; Calyplogena soyoae
Funding
- KAKENHI [21860097]
- Grants-in-Aid for Scientific Research [21860097, 22570105] Funding Source: KAKEN
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We describe a detailed structure determination of biosurfactant produced by Pseudozyina hubeiensis SY62, which was newly isolated from Calyptogena soyoae (deep-sea cold-seep clam, Shirouri-gai) at 1156 m in Sagami bay. P. hubeiensis SY62 was taxonomically slightly different from the P. hubeiensis type strain, which produces biosurfactants. Glycolipid production by the strain was also slightly different from those of previously reported strains. BS productivity was estimated to be around 30 g/l from the weight of the crude extract. At least five different spots of glycolipid biosurfactants (BSs) were detected by TLC. Results of nuclear magnetic resonance spectroscopies indicated the major product, namely MEL-C (4-O-[4'-O-acetyl-2',3'-di-O-alka(e) noil-beta-D-mannopyranosyl]-D-erythritol), as a promising BS. By further structural determination, the major fatty acids of MEL-C were estimated to be saturated C-6, C-10, and C-12 acids, which were shorter than those of previously reported MEL-C. Furthermore, H-1-NMR spectra implied the presence of C-2 acids as acyl groups. According to surface tension determination, the novel MEL-C showed larger critical micelle concentration (1.1 x 10(-)5 M) than conventional MEL-C which bound C-10 and C-12 acids (9.1 x 10(-6) M). From these results, shorter fatty acids would confer hydrophilicity onto the novel MEL-C. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.
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