Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 110, Issue 1, Pages 87-93Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2010.01.004
Keywords
Nuclear magnetic resonance (NMR); Stable-isotope probing (SIP); Metabolic dynamics; Carbon flux; Denaturing gradient gel electrophoresis (DGGE)
Funding
- J.S.P.S. [21247010]
- Grants-in-Aid for Scientific Research [21247010] Funding Source: KAKEN
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We have developed a new approach for monitoring the metabolic dynamics in microbial ecosystems using a combination of DNA fingerprinting and metabolome analysis based on stable-isotope-labeling technologies. Stable-isotope probing of DNA (DNA-SIP) has been used previously for the evaluation of cross-feeding in microbial communities. For the development and validation of our monitoring approach, fecal microbiota were analyzed with stable-isotope-labeled glucose used as the sole carbon source. In order to link the metabolic information and the microbial variability, we performed metabolic-microbial correlation analysis based on nuclear magnetic resonance (NMR) profiles and denaturing gradient gel electrophoresis (DGGE) fingerprints, which successfully identified the glucose-utilizing bacteria and their related extracellular metabolites. Moreover, our approach revealed information regarding the carbon flux, in that the first wave of extracellular metabolites secreted by the glucose-utilizing bacteria were incorporated into the secondary group of substrate-utilizing bacteria, and that this secondary group further produced their own secondary metabolized substrates. Thus, this approach is a powerful tool for monitoring the metabolic dynamics in microbial ecosystems and allows for the tracking of the carbon flux within a microbial community. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.
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