4.4 Article

Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP+-dependent xylitol dehydrogenase, and xylulokinase

Journal

JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 105, Issue 3, Pages 296-299

Publisher

SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1263/jbb.105.296

Keywords

lignocellulosic biomass; fermentation; ethanol; xylose; yeast; xylitol dehydrogenase; xylulokinase

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We constructed a set of recombinant Saccharomyces cerevisiae strains with xylose-fermenting ability. A recombinant S. cerevisiae strain D-XR/ARSdR/XK, in which protein engineered NADP(+)-dependent XDH was expressed, showed 40% increased ethanol production and 23% decrease in xylitol excretion as compared with the reference strain D-XR/XDH/XK expressing the wild-type XDH.

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