Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 106, Issue 6, Pages 610-613Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1263/jbb.106.610
Keywords
corneal endothelial cells; vitrification; cryoprotectant; cell monolayer
Funding
- National Science Foundation of China [2002008]
- Dalian Science and Technology Bureau [2005E11SF068]
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An in vitro model was developed for bovine natural corneal endothelia. The cells were cultured to a confluent monolayer and vitrified using 25% (w/w) 1,2-propanediol-35% (w/w) trehalose as cryoprotective agents. Approximately, 61.3% of the cells were viable using the protocol.
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