Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 105, Issue 6, Pages 628-635Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1263/jbb.105.628
Keywords
dibenzofuran; dioxin; biodegradation; ring-hydroxylating dioxygenase; ring-cleavage dioxygenase; hydrolase
Funding
- Ministry of Education, Culture, Sports, Science and Technology Japan [18780052]
- Ministry of Agriculture, Forestry,and Fisheries of Japan [HC-05-2322-1]
- Grants-in-Aid for Scientific Research [18780052] Funding Source: KAKEN
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A dibenzofuran (DF)-degrading bacterium, Nocardioides sp. DF412, was isolated and its genes responsible for the degradation of DF were cloned and characterized. The dfdA1A2A3A4 gene cluster coding for a ring hydroxylating dioxygenase was located on a plasmid, and the dfdBC gene cluster coding for a ring-cleavage dioxygenase and a following hydrolase respectively was located on the chromosome. Reverse transcription-PCR analysis revealed that the transcription of both gene clusters was induced in the presence of DF. These two gene clusters also conferred the activity of each enzyme to a host strain. These results strongly suggest that the clusters are responsible for the degradation of DF in strain DF412.
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