Journal
JOURNAL OF BIOPHOTONICS
Volume 8, Issue 6, Pages 441-456Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201400051
Keywords
hyperspectral imaging; multispectral imaging; biomedical imaging
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Funding
- National Institutes of Health (NIH) [EY015520, EY021470]
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Rather than simply acting as a photographic camera capturing two-dimensional (x, y) intensity images or a spectrometer acquiring spectra (lambda), a hyperspectral imager measures entire three-dimensional (x, y, lambda) datacubes for multivariate analysis, providing structural, molecular, and functional information about biological cells or tissue with unprecedented detail. Such data also gives clinical insights for disease diagnosis and treatment. We summarize the principles underpinning this technology, highlight its practical implementation, and discuss its recent applications at microscopic to macroscopic scales.
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