Journal
JOURNAL OF BIOPHOTONICS
Volume 8, Issue 1-2, Pages 52-59Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201300162
Keywords
optical tomography; velocimetry; microscopy; zebrafish; cardiovascular system
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Funding
- Cariplo Foundation [2009-2626]
- Adriano Buzzati-Traverso Foundation
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Microscopy techniques can readily visualize the finest details of embryo vasculature, but still lack to provide a complete three-dimensional representation of blood flow parameters. We present an in-vivo 3D imaging technique, able to reconstruct the blood cell velocity vector over a large volume of zebrafish embryos. This low cost and relatively simple technique is exploited to quantitatively assess blood velocity in the zebrafish tail at different stages of development.
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