Journal
JOURNAL OF BIOPHOTONICS
Volume 6, Issue 4, Pages 338-351Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201200079
Keywords
plasmonic nanoparticles; nanocomposites; photodynamic therapy; hematoporphyrin; Staphylococcus aureus
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Funding
- Russian Foundation for Basic Research [12-02-00379-a, 11-02-00128-a]
- Presidium of the Russian Academy of Sciences
- (Support for the Leading Scientific Schools) from the President of the Russian Federation [MK-1057.11.02, 1177.2012.2]
- Ministry of Science and Education of the Russian Federation [1.4.09, 02.740.11.0879]
- FP7-ICT-2007-2 [224014, PHOTONICS4LIFE]
- FiDiPro
- TEKES Program, Finland [40111/11]
- SCOPES EC, Switzerland/RF/Uzbekistan [2011-14]
- OPTEC company for supporting young scientists of leading higher educational institutions and scientific research centers
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We fabricated composite nanoparticles consisting of a plasmonic core (gold nanorods or goldsilver nanocages) and a hematoporphyrin-doped silica shell. The dual photodynamic and photothermal activities of such nanoparticles against Staphylococcus aureus 209 P were studied and compared with the activities of reference solutions (hematoporphyrin or silica-coated plasmonic nanoparticles). Bacteria were incubated with nanocomposites or with the reference solutions for 15 min, which was followed by CW light irradiation with a few exposures of 5 to 30 min. To stimulate the photodynamic and photothermal activities of the nanocomposites, we used LEDs (405 and 625 nm) and a NIR laser (808 nm), respectively. We observed enhanced inactivation of S. aureus 209 P by nanocomposites in comparison with the reference solutions. By using fluorescence microscopy and spectroscopy, we explain the enhanced antimicrobial effect of hematoporphyrin-doped nanocomposites by their selective accumulation in the vicinity of the bacteria. ((c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
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