Journal
JOURNAL OF BIOPHOTONICS
Volume 6, Issue 1, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201200146
Keywords
Raman spectroscopy; infrared spectroscopy; cardiovascular disease; arteries
Categories
Funding
- European Union
- European fund for regional development (EFRE)
- Thuringisches Ministerium fur Bildung, Wissenschaft und Kultur [B714-07037]
- Interdisziplinares Zentrum fur Klinische Forschung (IZKF) Jena
- network for excellence Photonics4Life
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Spectroscopy-based imaging techniques can provide useful biochemical information about tissue samples. Here, we employ Raman and Fourier transform infrared (IR) imaging to characterize composition and constitution of atherosclerotic plaques of rabbits, fed with a high cholesterol diet. The results were compared with conventional light microscopy after staining with hematoxylin eosin, and elastica van Gieson. The spectral unmixing algorithm vertex component analysis was applied for data analysis and image reconstruction. IR microscopy allowed for differentiation between lipids and proteins in plaques of full aortic cross sections. Raman microscopy further discriminated cholesterol esters, cholesterol and triglycerides. FTIR and Raman images were recorded at a resolution near 20 micrometer per pixel for a large field of view. High resolution Raman images at 1 micrometer per pixel revealed structural details at selected regions of interest. The intima-media and the lipid-protein ratio were determined in five specimens for quantitation. These results correlate well with histopathology. The described method is a promising tool for easy and fast molecular imaging of atherosclerosis.
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