Imaging of tumor vasculature using Twente photoacoustic systems

Photoacoustic imaging is a hybrid imaging modality based on the detection of acoustic waves generated by the absorption of short laser pulses in biological tissue. It combines the advantages of excellent contrast achieved in optical techniques with the high resolution of ultrasound imaging. In this article we present a review of the work done at the University of Twente to image tumor angiogenesis in vivo using this technique. We start with a description and the technical details of the different photoacoustic systems developed in our laboratory, with their validation on phantoms. We then discuss small-animal studies with results of serial imaging of angiogenesis over a 10-day period at the site of tumor induction in a rat. Further, we present clinical results using a photoacoustic mammoscope of breast cancer imaging based on angiogenesis-driven optical absorption contrast. [GRAPHICS] Photoacoustic image showing a ring-shaped area of higher intensity indicating vascularization associated with a cancer. This is a slice of the three-dimensional reconstructed data set of the imaged area, at a depth of 12 mm from the breast surface. (C) 2009 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim