Journal
JOURNAL OF BIOPHOTONICS
Volume 2, Issue 12, Pages 725-735Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.200910078
Keywords
circulating tumor cells; cancer stem cells; photoacoustic method; photothermal therapy; in-vivo flow cytometry; early cancer diagnosis
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Funding
- National Institute of Health [R01EB000873, R01CA131164, R01 EB009230, R21CA139373]
- National Science Foundation [DBI-0852737, CMMI-0709121]
- Arkansas Biosciences Institute
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0852737] Funding Source: National Science Foundation
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In-vivo multicolor photoacoustic (PA) flow cytometry for ultrasensitive molecular detection of the CD44+ circulating tumor cells (CTCs) is demonstrated on a mouse model of human breast cancer. Targeting of CTCs with stem-like phenotype, which are naturally shed from parent tumors, was performed with functionalized gold and magnetic nanoparticles. Results in vivo were verified in vitro with a multifunctional microscope, which integrates PA, photothermal (PT), fluorescent and transmission modules. Magnet-induced clustering of magnetic nanoparticles in individual cells significantly amplified PT and PA signals. The novel noninvasive platform, which integrates multispectral PA detection and PT therapy with a potential for multiplex targeting of many cancer biomarkers using multicolor nanoparticles, may prospectively solve grand challenges in cancer research for diagnosis and purging of undetectable yet tumor-initiating cells in circulation before they form metastasis. [GRAPHICS] Integrated fluorescent and transmission image of mouse blood spiked with breast cancer cells, which were prior labeled with FITC. (C) 2009 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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