Journal
JOURNAL OF BIOPHOTONICS
Volume 3, Issue 1-2, Pages 44-52Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.200910080
Keywords
tissue optical-clearing technique; in vivo rat skin; glycerol; mechanism
Categories
Funding
- National Nature Science Foundation of China [30770552]
- NSFC-RFBR for International Cooperation [30911120074]
- Ministry of Education and State Administration of Foreign Experts Affairs of China
- RFBR-NSF China [08-02-92224-a]
- RF Program on the Development of High School Potential [2.1.1/4989, 2.2.1.1/2950]
- RF [208.2008.2]
- Federal Agency of Education of RF [1.4.09]
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The penetration of light in biological tissues can be enhanced by using optical-clearing techniques. However, researches on the mechanism are limited to in vitro experiments. In this study, rat dorsal skin was used to discuss the mechanism for in vivo optical clearing. Glycerol solutions with different concentrations were applied by dermal injection; then the skin reflectance spectrum, SHG imaging and microstructural changes were monitored. The results showed that with the skin becoming transparent, the corresponding reflectance decreased, and the thickness of dermis and diameter of collagen fibril decreased, but no collagen fiber was dissolved or fractured. Hence, it can be concluded that the thickness decrease of dermis and corresponding more regular packing of tissue fibers plays an important role in the mechanism for glycerol-induced optical clearing of skin in vivo. [GRAPHICS] Images of dermal collagen fibers taken by TEM 10 min after injection of (a) blank control; (b) 20%-glycerol; (c) 30%-glycerol; (d) 75%-glycerol; (x30000, scale = 333 nm). (C) 2010 by WILEY-VCH Verlag GmbH & Co. KGaA. Weinheim
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