Journal
JOURNAL OF BIOPHOTONICS
Volume 1, Issue 3, Pages 183-199Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.200810011
Keywords
Femtosecond; laser; transfection; photoporation; Bessel beam; biophotonics
Categories
Funding
- Engineering and Physical Sciences Research Council (EPSRC)
- EPSRC [EP/E06440X/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/E06440X/1] Funding Source: researchfish
Ask authors/readers for more resources
In this work we present a review and discussion on the enhancement of femtosecond (fs) laser for use within biophotonics with a particular focus on their use in optical transfection techniques. We describe the broad range of source options now available for the generation of femtosecond pulses before briefly reviewing the application of fs laser in optical transfection studies. We show that major performance enhancements may be obtained by optimising the spatial and temporal performance of the laser source before considering possible future directions in this fields. In relation to optical transfection we describe how such laser source initiate a multiphoton process to permeate the cell membrane in a transient fashion. We look at aspects of this technique including the ability to combine transfection of such transfection we explore the role of new sources and nondiffracting light fields. (C) 2008 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available