Journal
JOURNAL OF BIOPHOTONICS
Volume 1, Issue 5, Pages 419-424Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.200810015
Keywords
Raman microspectroscopy; polarization-resolved; keratin; human hair; amide I; band decomposition; secondary structure; orientation
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Polarization-resolved Raman microspectroscopy with near-infrared laser excitation was applied to intact human hair in order to non-invasively investigate the conformation and orientation of the polypeptide chains. By varying the orientation of the hair shaft relative to the polarization directions of the laser/analyzer, a set of four polarized Raman spectra is obtained; this allows to simultaneously determine both the secondary structure of hair proteins and the orientation of the polypeptide strands relative to the axis of the hair shaft. For the amide 1 band, results from a quantitative analysis of the polarized Raman spectra are compared with theoretically expected values for fibers with uniaxial symmetry. Based on the polarization behavior of the amide 1 band and further vibrational bands, a partial ordering of alpha-helical polypeptide strands parallel to the hair shaft can be concluded. We suggest that this microspectroscopic approach may be used for human hair diagnostics by detecting structural or orientational alterations of keratins.
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