Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 60, Issue 2-3, Pages 157-168Publisher
SPRINGER
DOI: 10.1007/s10858-014-9865-8
Keywords
NMR; Dynamic nuclear polarization; Membrane; Protein; Solid-state NMR
Categories
Funding
- NWO [722.012.002, 700.11.344, 700.58.102]
- DFG [Po137, 40-1, 41-1]
- NIH (NIH/NIGNS) [GM087519]
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Dynamic nuclear polarization (DNP) has become a powerful method to enhance spectroscopic sensitivity in the context of magnetic resonance imaging and nuclear magnetic resonance spectroscopy. We show that, compared to DNP at lower field (400 MHz/263 GHz), high field DNP (800 MHz/527 GHz) can significantly enhance spectral resolution and allows exploitation of the paramagnetic relaxation properties of DNP polarizing agents as direct structural probes under magic angle spinning conditions. Applied to a membrane-embedded K+ channel, this approach allowed us to refine the membrane-embedded channel structure and revealed conformational substates that are present during two different stages of the channel gating cycle. High-field DNP thus offers atomic insight into the role of molecular plasticity during the course of biomolecular function in a complex cellular environment.
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