Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 52, Issue 4, Pages 329-337Publisher
SPRINGER
DOI: 10.1007/s10858-012-9613-x
Keywords
Intrinsically disordered proteins; Non-uniform sampling; Backbone assignment; 5D NMR; 6D NMR
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Funding
- EC [261863]
- Foundation for Polish Science
- EU
- Czech Science Foundation [204/09/0583]
- [AV0Z50200510]
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Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using delta subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences.
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