Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 50, Issue 3, Pages 237-245Publisher
SPRINGER
DOI: 10.1007/s10858-011-9515-3
Keywords
P-31 NMR chemical shift correlation; O-18/O-16 isotopic effects; ATP turnover; Isotopologue distribution; Oxygen-18; High-energy phosphoryl dynamics; Phosphotransfer; Phosphometabolomics
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Funding
- National Institutes of Health
- Marriott Heart Disease Research Program
- Marriott Foundation
- Mayo Clinic
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Intramolecular correlations among the O-18-labels of metabolic oligophosphates, mapped by J-decoupled P-31 NMR 2D chemical shift correlation spectroscopy, impart stringent constraints to the O-18-isotope distributions over the whole oligophosphate moiety. The multiple deduced correlations of isotopic labels enable determination of site-specific fractional isotope enrichments and unravel the isotopologue statistics. This approach ensures accurate determination of O-18-labeling rates of phosphometabolites, critical in biochemical energy conversion and metabolic flux transmission. The biological usefulness of the J-decoupled P-31 NMR 2D chemical shift correlation maps was validated on adenosine tri-phosphate fractionally O-18 labeled in perfused mammalian hearts.
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