Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 44, Issue 3, Pages 157-166Publisher
SPRINGER
DOI: 10.1007/s10858-009-9325-z
Keywords
Lanthanide binding peptide tag; Two-point anchoring; Paramagnetic NMR; Pseudo-contact shift; Residual dipolar coupling
Categories
Ask authors/readers for more resources
Paramagnetic lanthanide ions fixed in a protein frame induce several paramagnetic effects such as pseudo-contact shifts and residual dipolar couplings. These effects provide long-range distance and angular information for proteins and, therefore, are valuable in protein structural analysis. However, until recently this approach had been restricted to metal-binding proteins, but now it has become applicable to non-metalloproteins through the use of a lanthanide-binding tag. Here we report a lanthanide-binding peptide tag anchored via two points to the target proteins. Compared to conventional single-point attached tags, the two-point linked tag provides two to threefold stronger anisotropic effects. Though there is slight residual mobility of the lanthanide-binding tag, the present tag provides a higher anisotropic paramagnetic effect.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available