Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 42, Issue 1, Pages 59-67Publisher
SPRINGER
DOI: 10.1007/s10858-008-9264-0
Keywords
amino acid selective isotope labeling; intrinsically disordered protein; Neh2; N-15-glutamate labeling; N-15-glutamine labeling
Categories
Funding
- JSTERATO
- Ministry of Education, Culture, Sports, Science, and Technology
Ask authors/readers for more resources
A simple and user-friendly method of labeling protein selectively with amino acids in vivo is introduced. This technique does not require the use of transaminase-deficient or auxotrophic strains. By manipulating the product feedback inhibitory loops of the E. coli amino acid metabolic pathways and, if necessary, by using enzyme inhibitors, proteins were labeled efficiently in vivo even with amino acid types that are central to the metabolic pathways, such as glutamine. The sequential backbone resonance assignment of the Neh2 domain of Nrf2 transcriptional factor, an intrinsically disordered protein with high spectral degeneracy, was achieved using this labeling method.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available