4.1 Article

CD73 Is Critical for the Resolution of Murine Colonic Inflammation

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HINDAWI LTD
DOI: 10.1155/2012/260983

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  1. National Institutes of Health [A1072434-A2, R01NS063011]

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CD73 is a glycosyl-phosphatidylinositol-(GPI-) linked membrane protein that catalyzes the extracellular dephosphorylation of adenosine monophosphate (AMP) to adenosine. Adenosine is a negative regulator of inflammation and prevents excessive cellular damage. We investigated the role of extracellular adenosine in the intestinal mucosa during the development of Dextran-Sulfate-Sodium-(DSS-) salt-induced colitis in mice that lack CD73 (CD73(-/-)) and are unable to synthesize extracellular adenosine. We have found that, compared to wild-type (WT) mice, CD73(-/-) mice are highly susceptible to DSS-induced colitis. CD73(-/-) mice exhibit pronounced weight loss, slower weight recovery, an increase in gut permeability, a decrease in expression of tight junctional adhesion molecules, as well as unresolved inflammation following the removal of DSS. Moreover, colonic epithelia in CD73(-/-) mice exhibited increased TLR9 expression, high levels of IL-1 beta and TNF-alpha, and constitutive activation of NF-kappa B. We conclude that CD73 expression in the colon is critical for regulating the magnitude and the resolution of colonic immune responses.

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