Journal
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
Volume -, Issue -, Pages -Publisher
HINDAWI PUBLISHING CORPORATION
DOI: 10.1155/2010/290286
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Funding
- Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Spain [CAL01-045-C2-2]
- Xunta de Galicia, Spain [PGIDT00BIO1E]
- Ministerio de Educacion y Ciencia [MAT2005-05393-C03-03, MAT2006-11662-C03-03]
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The influence of initial pHon growth and nutrient (total sugars, nitrogen, and phosphorous) consumption by Enterococcus faecium CECT 410 was studied during batch cultures in whey. With these data, two realkalized fed-batch fermentations were developed using different feeding substrates. The shift from homolactic to mixed acid fermentation, the biphasic kinetics observed for cell growth and nitrogen consumption and the increase in the concentrations of biomass and products (lactic acid, acetic acid, ethanol, and butane-2,3-diol) were the most noteworthy observations of these cultures. Modelling the fed-batch growth of Ent. faecium with the Logistic and bi-Logisticmodels was not satisfactory. However, biomass production was best mathematically described with the use of a double Monod model, which was expressed in terms of biomass, product accumulation, and nitrogen utilization. Product formation was successfully modelled with a modified form of the Luedeking and Piret model developed in this study.
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