Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 19, Issue 10, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.19.10.106002
Keywords
microscopy; light-emitting diode array; phase contrast; real time; brightfield; darkfield
Funding
- Development Impact Lab (USAID) part of USAID's Higher Education Solutions Network [AID-OAA-A-13-00002, AID-OAA-A-12-00011]
- Office of Naval Research [N00014-14-1-0083]
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We demonstrate a single-camera imaging system that can simultaneously acquire brightfield, darkfield, and phase contrast images in real time. Our method uses computational illumination via a programmable light-emitting diode (LED) array at the source plane, providing flexible patterning of illumination angles. Brightfield, darkfield, and differential phase contrast images are obtained by changing the LED patterns, without any moving parts. Previous work with LED array illumination was only valid for static samples because the hardware speed was not fast enough to meet real-time acquisition and processing requirements. Here, we time multiplex patterns for each of the three contrast modes in order to image dynamic biological processes in all three contrast modes simultaneously. We demonstrate multicontrast operation at the maximum frame rate of our camera (50 Hz with 2160 x 2560 pixels). (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.
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