Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 19, Issue 4, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.19.4.046022
Keywords
digital holographic microscopy; quantitative phase imaging; aberration compensation
Funding
- Ministerio de Economia y Competitividad, Spain [DPI2012-32994]
- GeneralitatValenciana [PROMETEO2009-077]
- University of Valencia through the predoctoral fellowship program Atraccio de Talent
- University of Valencia, Spain under the program Estancias Temporales para Investigadores Invitados, Colciencias [110205024]
- UNAL [110201003, 110201004]
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The advantages of using a telecentric imaging system in digital holographic microscopy (DHM) to study biological specimens are highlighted. To this end, the performances of nontelecentric DHM and telecentric DHM are evaluated from the quantitative phase imaging (QPI) point of view. The evaluated stability of the microscope allows single-shot QPI in DHM by using telecentric imaging systems. Quantitative phase maps of a section of the head of the drosophila melanogaster fly and of red blood cells are obtained via single-shot DHM with no numerical postprocessing. With these maps we show that the use of telecentric DHM provides larger field of view for a given magnification and permits more accurate QPI measurements with less number of computational operations. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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