4.5 Article

Surface plasmon resonance-based infrared biosensor for cell studies with simultaneous control

Journal

JOURNAL OF BIOMEDICAL OPTICS
Volume 19, Issue 11, Pages -

Publisher

SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.19.11.111608

Keywords

sensors; surface plasmons; waveguides; cells; infrared spectroscopy; Fourier transform spectroscopy

Funding

  1. Yissum Research and Development Company of the Hebrew University of Jerusalem

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We report a label-free infrared surface plasmon biosensor with a double-chamber flow cell for continuous monitoring of morphological changes in cell culture exposed to various stimuli. In this technique, the monolayer of cultured cells is divided into two halves by a barrier, allowing the treatment of one half while the other serves as control. We demonstrate the advantages of this setup in test experiments that track kinetics of the IEC-18 cell layer response to variations in extracellular Ca2+ concentration. The sensitivity of the presented method was found to be an order of magnitude higher compared to the single-chamber biosensor. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)

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