Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 17, Issue 8, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.17.8.080505
Keywords
optical-resolution photoacoustic microscopy; fast scanning; MEMS scanning mirror; blood flow dynamics; confocality; confocal sensitivity
Funding
- NIH [R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, R01 CA159959]
- NSF [CMMI-1131758]
- Div Of Civil, Mechanical, & Manufact Inn
- Directorate For Engineering [1131758] Funding Source: National Science Foundation
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By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have to sacrifice either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible microelectromechanical systems (MEMS) scanning mirror (MEMS-OR-PAM). In MEMS-OR-PAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures the uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. Presented results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.8.080505]
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