Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 17, Issue 11, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.17.11.116001
Keywords
fluorescence microscopy; molecular probe; near infrared; polarization microscopy; surgical margin; tumor imaging
Funding
- National Institutes of Health [NIBIB R01 EB008111, NCI R01 CA171651]
- Air Force Office of Scientific Research [FA9550-10-1-0121, FA9550-12-1-0321]
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Fluorescence microscopy offers high sensitivity for disease diagnosis. However, little structural information is revealed by this method, requiring another technique to localize the source of fluorescence. We developed a complementary fluorescence-polarization microscope. We used a division-of-focal-plane charge coupled device polarization sensor to enable real-time video rate polarization imaging without any moving parts. The polarization information provided by the microscope enabled detection of structural element and complements the fluorescence information. Application of this multimodal system for cancer imaging using a tumor selective molecular probe revealed the association of diminished structural integrity of tumor tissue with high fluorescence of the imaging agent compared to surrounding normal tissue. This study demonstrates a new paradigm to improve cancer detection and diagnosis. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.11.116001]
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