Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 17, Issue 5, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.17.5.056016
Keywords
interferometry; photoacoustic; preclinical; tumor
Funding
- King's College London
- University College London Comprehensive Cancer Imaging Centre Cancer Research UK
- Medical Research Council and Department of Health, UK
- Engineering and Physical Sciences Research Council
- EPSRC [EP/E050980/1, EP/H005536/1] Funding Source: UKRI
- MRC [G1001497] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/H005536/1, EP/E050980/1] Funding Source: researchfish
- Medical Research Council [G1001497] Funding Source: researchfish
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The use of a novel all-optical photoacoustic scanner for imaging the development of tumor vasculature and its response to a therapeutic vascular disrupting agent is described. The scanner employs a Fabry-Perot polymer film ultrasound sensor for mapping the photoacoustic waves and an image reconstruction algorithm based upon attenuation-compensated acoustic time reversal. The system was used to noninvasively image human colorectal tumor xenografts implanted subcutaneously in mice. Label-free three-dimensional in vivo images of whole tumors to depths of almost 10 mm with sub-100-micron spatial resolution were acquired in a longitudinal manner. This enabled the development of tumor-related vascular features, such as vessel tortuosity, feeding vessel recruitment, and necrosis to be visualized over time. The system was also used to study the temporal evolution of the response of the tumor vasculature following the administration of a therapeutic vascular disrupting agent (OXi4503). This revealed the well-known destruction and recovery phases associated with this agent. These studies illustrate the broader potential of this technology as an imaging tool for the preclinical and clinical study of tumors and other pathologies characterized by changes in the vasculature. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.5.056016]
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