Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 16, Issue 3, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3556717
Keywords
red blood cells; erythrocytes; sickle cell disease; sickle cell anemia; cell imaging; quantitative phase microscopy; interferometry
Funding
- National Science Foundation (NSF) [CBET-0651622, MRI-1039562]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1039562] Funding Source: National Science Foundation
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We have applied wide-field digital interferometry (WFDI) to examine the morphology and dynamics of live red blood cells (RBCs) from individuals who suffer from sickle cell anemia (SCA), a genetic disorder that affects the structure and mechanical properties of RBCs. WFDI is a noncontact, label-free optical microscopy approach that can yield quantitative thickness profiles of RBCs and measurements of their membrane fluctuations at the nanometer scale reflecting their stiffness. We find that RBCs from individuals with SCA are significantly stiffer than those from a healthy control. Moreover, we show that the technique is sensitive enough to distinguish classes of RBCs in SCA, including sickle RBCs with apparently normal morphology, compared to the stiffer crescent-shaped sickle RBCs. We expect that this approach will be useful for diagnosis of SCA and for determining efficacy of therapeutic agents. (C) 2011 society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3556717]
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