Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 16, Issue 5, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3582335
Keywords
Mohs surgery; confocal fluorescence mosaicing microscopy; basal cell carcinoma; surgical pathology; large area imaging microscopy
Funding
- NIH
- NIBIB [R01EB012466]
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Imaging large areas of tissue rapidly and with high resolution may enable rapid pathology at the bedside. The limited field of view of high-resolution microscopes requires the merging of multiple images that are taken sequentially to cover a large area. This merging or mosaicing of images requires long acquisition and processing times, and produces artifacts. To reduce both time and artifacts, we developed a mosaicing method on a confocal microscope that images morphology in large areas of excised tissue with sub-cellular detail. By acquiring image strips with aspect ratios of 10:1 and higher (instead of the standard similar to 1:1) and stitching them in software, our method images 10x10 mm(2) area of tissue in about 3 min. This method, which we call strip mosaicing, is currently three times as fast as our previous method. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3582335]
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