Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 16, Issue 4, Pages -Publisher
SPIE-SOC PHOTOPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3569614
Keywords
nonlinear microscopy; adaptive optics; three-dimensional microscopy; medical and biological imaging
Funding
- BBSRC [BB/F011512/1]
- EPSRC [EP/E055818/1]
- Austrian Science Fund [J2826-N20]
- Wellcome Trust [074246/Z04/Z]
- BBSRC [BB/E004946/1, BB/F011512/1] Funding Source: UKRI
- EPSRC [EP/E055818/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F011512/1, BB/E004946/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/E055818/1] Funding Source: researchfish
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We present a detailed description of an adaptive harmonic generation (HG) microscope and culture techniques that permit long-term, three-dimensional imaging of mouse embryos. HG signal from both pre- and postimplantation stage (0.5-5.5 day-old) mouse embryos are fully characterized. The second HG images reveal central spindles during cytokinesis whereas third HG images show several features, such as lipid droplets, nucleoli, and plasma membranes. The embryos are found to develop normally during one-day-long discontinuous HG imaging, permitting the observation of several dynamic events, such as morula compaction and blastocyst formation. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3569614]
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