Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 15, Issue 5, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3486612
Keywords
fluorescence lifetime imaging microscopy; tissue autofluorescence; endoscopy; malignant glioma; brain tumors; intraoperative cancer diagnosis
Funding
- University of California Davis Cancer Center
- NIH [HL067377, RO1 NS40489, R01 NS060880]
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We demonstrate for the first time the application of an endoscopic fluorescence lifetime imaging microscopy (FLIM) system to the intraoperative diagnosis of glioblastoma multiforme (GBM). The clinically compatible FLIM prototype integrates a gated (down to 0.2 ns) intensifier imaging system with a fiber-bundle (fiber image guide of 0.5 mm diameter, 10,000 fibers with a gradient index lens objective 0.5 NA, and 4 mm field of view) to provide intraoperative access to the surgical field. Experiments conducted in three patients undergoing craniotomy for tumor resection demonstrate that FLIM-derived parameters allow for delineation of tumor from normal cortex. For example, at 460 +/- 25-nm wavelength band emission corresponding to NADH/NADPH fluorescence, GBM exhibited a weaker florescence intensity (35% less, p-value <0.05) and a longer lifetime tau(GBM-Amean) = 1.59 +/- 0.24 ns than normal cortex tau(NC-Amean) = 1.28 +/- 0.04 ns (p-value <0.005). Current results demonstrate the potential use of FLIM as a tool for image-guided surgery of brain tumors. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3486612]
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