Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 15, Issue 1, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3299321
Keywords
diffuse optics; near infrared; photon migration; spatial modulation
Funding
- NIH [P41-RR01192]
- National Cancer Institute [R21-CA129758]
- Beckman Foundation
- AFOSR [FA9550-08-1-0384]
- NATIONAL CANCER INSTITUTE [P30CA062203, R21CA129758] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR001192] Funding Source: NIH RePORTER
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We introduce a noncontact imaging method utilizing multifrequency structured illumination for improving lateral and axial resolution and contrast of fluorescent molecular probes in thick, multiple-scattering tissue phantoms. The method can be implemented rapidly using a spatial light modulator and a simple image demodulation scheme similar to structured light microscopy in the diffraction regime. However, imaging is performed in the multiple-scattering regime utilizing spatially modulated scalar photon density waves. We demonstrate that by increasing the structured light spatial frequency, fluorescence from deeper structures is suppressed and signals from more superficial objects enhanced. By measuring the spatial frequency dependence of fluorescence, background can be reduced by localizing the signal to a buried fluorescent object. Overall, signal-to-background ratio (SBR) and resolution improvements are dependent on spatial frequency and object depth/dimension with as much as sevenfold improvement in SBR and 33% improvement in resolution for similar to 1-mm objects buried 3 mm below the surface in tissue-like media with fluorescent background. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3299321]
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