Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 15, Issue 1, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3290243
Keywords
Candida albicans; fluorescence; confocal microscopy; in vivo imaging
Funding
- NIH National Center for Research Resources [ULI RR02160]
- National Cancer Institute [CA68409, CA122093]
- NATIONAL CANCER INSTITUTE [R01CA122093, R01CA068409] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR RESEARCH RESOURCES [KL2RR024136] Funding Source: NIH RePORTER
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Candida albicans is an opportunistic human fungal pathogen that requires an intact host immune response to prevent disease. Thus, studying host-pathogen interactions is critical to understanding and preventing this disease. We report a new model infection system in which ongoing C. albicans infections can be imaged at high spatial resolution in the ears of living mice. Intradermal inoculation into mouse ears with a C. albicans strain expressing green fluorescent protein results in systemic C. albicans infection that can be imaged in vivo using confocal microscopy. We observed filamentous growth of the organism in vivo as well as formation of microabscesses. This model system will allow us to gain significant new information about C. albicans pathogenesis through studies of host-C. albicans interactions in the native environment. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3290243]
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