Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 15, Issue 4, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3465594
Keywords
aptamer; quadruplex; label-free assay; direct detection; thrombin; surface-enhanced Raman Scattering
Funding
- National Science Foundation (NSF)
- Lawrence Livermore National Laboratory (LLNL)
- LLNL-JRNL [LLNL-JRNL-421298-DRAFT]
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In this study, we exploit the sensitivity offered by surface-enhanced Raman scattering (SERS) for the direct detection of thrombin using the thrombin-binding aptamer (TBA) as molecular receptor. The technique utilizes immobilized silver nanoparticles that are functionalized with thiolated thrombin-specific binding aptamer, a 15-mer (5'-GGTTGGTGTGGTTGG-3') quadruplex forming oligonucleotide. In addition to the Raman vibrational bands corresponding to the aptamer and blocking agent, new peaks (mainly at 1140, 1540, and 1635 cm(-1)) that are characteristic of the protein are observed upon binding of thrombin. These spectral changes are not observed when the aptamer-nanoparticle assembly is exposed to a nonbinding protein such as bovine serum albumin (BSA). This methodology could be further used for the development of label-free biosensors for direct detection of proteins and other molecules of interest for which aptamers are available. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3465594]
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