Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 15, Issue 3, Pages -Publisher
SPIE-SOC PHOTOPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3420179
Keywords
phase microscopy; cell imaging; myocardial cells; myocytes; euglena
Funding
- NSF [CBET-0651622]
- Bikura Postdoctoral Fellowship, Israel
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1039562] Funding Source: National Science Foundation
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We introduce a new interferometric setup for single-exposure wide-field holographic phase imaging of highly dynamic biological samples. In this setup, the interferometric signal originates from a specially designed reflective interferometric chamber (InCh), creating an off-axis interferogram on the output plane of the system. The setup only requires the InCh and a simple reflection-mode two lens imaging system, without the need for additional optical elements such as gratings in the beam path. In addition, due to the close-to-common-path geometry of the setup, phase noise is greatly reduced. We experimentally compare the inherent phase stability of the system in ambient conditions to that of a conventional interferometer. We also demonstrate use of this system for wide-field quantitative phase imaging of two different highly dynamic, optically transparent biological samples: beating myocardial cells and moving unicellular microorganisms. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3420179]
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